Wize igipimo cya firime ya kashe?
Niki?Ninde wundi udashobora "gufunga firime"?Ihute witonze kuriyi ngingo kugirango ikwigishe neza "firime ya kashe"!
Birumvikana ko "kashe ya firime" hano ari ugushiraho isahani 96 iriba PCR kugirango barebe ko firime ifunga neza ihuza isahani 96 kandi ikarinda guhumeka neza, kugirango igeragezwa ryoroshye.
1. Fata kashe ya kashe ku kibaho
Kuramo icyuma kimwe gifunga igikapu cyo kwifungisha, hanyuma ukureho igikapu cyo gufunga kugirango ugumane enzyme kubidukikije.Komeza umurongo wo hasi hejuru, ufate firime ya kashe, hanyuma usenye buhoro buhoro umurongo wo hasi kumurongo ugaragara.
Noneho, shyira ku mpera imwe yubuso bwa firime ifunga kashe, hanyuma ufate intera nu mpande kugirango wirinde gukurikira.Muburyo bwo gushira, impera imwe yarashizwemo indi mpera ikururwa.
Inama: Buri gihe wambare uturindantoki
● Niba firime yo gufunga ikirango cyanyuma ikoreshwa, kura umurongo igice, shyira kashe ya kashe kumubaho kugirango uyifungire ku kibaho cyose, hanyuma ukomeze gukuramo umurongo.Ubu buryo burashobora gukuraho gutembera no gusubira inyuma biterwa na firime ya kashe.
● Niba ibicuruzwa bifite ibirango bibiri byanyuma byakoreshejwe, kuramo umurongo wo hagati muburyo bukomeza kandi bworoshye.Kwambura buhoro umurongo bigabanya kugabanuka.Witondere kudakora ku buso bwa firime.
2. Kanda firime
Koresha icyapa cyumuvuduko kugirango usibe buhoro buhoro hanyuma ukande kashe ya plaque kugirango uyifungire neza kubisahani.Niba nta laminate idasanzwe, urashobora kubona ikarita ifite impande zoroshye, nk'ikarita ya banki cyangwa ikarita ya bisi.
Intambwe yo gukanda ya firime igomba gukorwa byibuze kabiri mu buryo butambitse kandi buhagaritse.Ni ngombwa gukoresha imbaraga zihagije kugirango ubone kashe nziza.
Kuraho hanyuma ukande membrane ikanda isahani kumpande zose zinyuma zicyapa cya orifice byibuze kabiri kugirango umenye neza ko igitutu gihamye kandi gihoraho.Imyobo n'impande bigomba gukanda rimwe.Nyuma yo gufunga firime neza neza ku isahani, kura igice cyahujwe kumurongo wa tangent.
Impanuro: ● Mugihe ukanda firime, fata ikibaho ukundi kuboko kugirango wirinde kunyeganyega bikabije.
3. Kugenzura
Nyuma yo gufunga, genzura neza isahani iringaniye kugirango umenye niba firime ifatanye neza nisahani.Emeza ko ibimenyetso bifatika kuri buri mwobo, ubuso bwose bw'isahani (harimo na peripheri) bifunze, kandi niba hari amazi kuri membrane.Filime ifunga kashe ntigomba kugira iminkanyari.Niba hagaragaye iminkanyari, isahani ntabwo ifunze neza.
● Kubisahani binini bifite impande zazamuye, umwanya wa firime ifunga kashe ntishobora kuba nziza, kandi firime ntishobora kwaguka hejuru kurukuta rwuruhande rwisahani.
Shira isahani ifunze byibuze iminota 10 mbere yo gutangira igeragezwa rya PCR, kandi imbaraga zifatika za firime zifunga iziyongera hamwe nigihe.Niba bishoboka, koresha centrifuge idasanzwe kuri plaque ya orifice kuri centrifugation.Hanyuma, ohereza isahani ifunze kumashini ya PCR kugirango utangire igeragezwa ~
Inama:
● Kubisahani binini bifite impande zazamuye, umwanya wa firime ifunga kashe ntishobora kuba nziza, kandi firime ntishobora kwaguka hejuru kurukuta rwuruhande rwisahani.
Shira isahani ifunze byibuze iminota 10 mbere yo gutangira igeragezwa rya PCR, kandi imbaraga zifatika za firime zifunga iziyongera hamwe nigihe.Niba bishoboka, koresha centrifuge idasanzwe kuri plaque ya orifice kuri centrifugation.Hanyuma, ohereza isahani ifunze kumashini ya PCR kugirango utangire igeragezwa ~
Igihe cyo kohereza: Ukuboza-16-2022