Inama ku guhitamo no gukoresha ibyapa byumuco w'akagari (I)
Nkigikoresho gisanzwe kandi cyingenzi mumico yutugari, isahani yumuco utugari ifite imiterere itandukanye, ibisobanuro nibikoreshwa.
Urayobewe kandi uburyo bwo guhitamo icyapa gikwiye?
Ufite impungenge zo gukoresha isahani yumuco byoroshye kandi neza?
Urayobewe uburyo bwo guhangana nicyapa cyumuco?
Wumva umeze ute kubijyanye no gukoresha neza isahani yumuco itandukanye?
Nigute ushobora guhitamo isahani yumuco?
1) Ibyapa byumuco w'akagari birashobora kugabanywa hepfo no hepfo (U-shusho na V-shusho) ukurikije imiterere yo hasi;
2) Umubare wimyobo yumuco yari 6, 12, 24, 48, 96, 384, 1536, nibindi;
3) Ukurikije ibikoresho bitandukanye, hariho isahani ya Terasaki hamwe nicyapa gisanzwe cyumuco.Guhitamo byihariye biterwa n'ubwoko bw'utugingo ngengabuzima, ingano y'umuco isabwa n'intego zitandukanye zigerageza.
Itandukaniro no guhitamo icyapa kizengurutse kandi kizengurutse (U-shusho na V-shusho)
Ubwoko butandukanye bwibibaho bisanzwe bifite imikoreshereze itandukanye
Ubwoko bwose bwa selile yo hasi irashobora gukoreshwa, ariko mugihe umubare wutugingo ngengabuzima ari muto, nka cloni, 96 neza neza isahani yo hasi.
Mubyongeyeho, mugihe ukora MTT nubundi bushakashatsi, isahani yo hasi isanzwe ikoreshwa mubisanzwe byombi bifatanye kandi byahagaritswe.
Kubijyanye na U-shusho cyangwa V-isahani, mubisanzwe bikoreshwa mubisabwa bidasanzwe.Kurugero, muri immunologiya, iyo lymphocytes ebyiri zitandukanye zivanze, bakeneye kuvugana kugirango bakangure.Kubwibyo, amasahani U-asabwa muri rusange.Kuberako selile zizateranira murwego ruto bitewe ningaruka za rukuruzi, isahani ya V ifite akamaro gake.Isahani ya V isanzwe ikoreshwa mubushakashatsi bwica selile kugirango selile igere hafi, ariko amasahani U-arashobora no gukoreshwa murubu bushakashatsi (nyuma yo kongeramo selile, centrifuge kumuvuduko muke).
Niba ikoreshwa mumico y'utugari, mubisanzwe iringaniye.Byongeye kandi, hakwiye kwitabwaho bidasanzwe ibikoresho.Ikimenyetso "Umuco wa Tissue (TC) Uvuwe" gikoreshwa mumico y'akagari.
Uruziga ruzengurutse rusanzwe rukoreshwa mu gusesengura, gufata imiti, cyangwa kubika icyitegererezo.Kuberako uruziga ruzengurutse aribyiza byo gukuramo amazi, kandi hasi ntabwo aribyo.Ariko, niba ushaka gupima agaciro ko kwinjiza urumuri, ugomba kugura hepfo imwe.
Imico myinshi yutugari ikoresha isahani yumuco wo hasi, byoroshye kuyibona munsi ya microscope, ifite ahantu hakeye, ifite uburebure bwimiterere yumuco utugari, kandi ikanorohereza kumenya MTT.
Isahani yumuco wo hepfo ikoreshwa cyane cyane mugupima isotope, kandi igikoresho cyo gukusanya ingirabuzimafatizo gisabwa gukusanya umuco w'akagari, nka "umuco wa lymphocyte uvanze".
Igihe cyo kohereza: Ukuboza-08-2022