Imiyoboro ya ultrafiltration centrifuge irashobora kongera gukoreshwa?Dore igisubizo

Umuyoboro wa centrifuge ni umuyoboro woroshye ushobora kwihanganira umuvuduko ukabije n’umuvuduko, nko gutandukanya ingero zimwe na zimwe no gutandukanya imyanda ndengakamere.Umuyoboro wa ultrafiltration centrifuge ufite ibice bibiri bisa numuyoboro w'imbere n'umuyoboro w'inyuma.Umuyoboro w'imbere ni membrane ifite uburemere runaka bwa molekile.Mugihe cyihuta cya centrifugation, abafite uburemere buke bwa molekile bazinjira mumiyoboro yo hepfo (ni ukuvuga umuyoboro winyuma), naho abafite uburemere bunini bwa molekile bazafatirwa mumiyoboro yo hejuru (ni ukuvuga umuyoboro w'imbere).Iri ni ihame rya ultrafiltration kandi akenshi rikoreshwa muguhuza ingero.

Imiyoboro ya Ultrafiltration centrifuge mubisanzwe irashobora gukoreshwa utabanje kwitegura, ariko mugutunganya intungamubiri za poroteyine, cyane cyane kubisubizo bya poroteyine (<10ug / ml), igipimo cyo gukira kwinshi hamwe na membrane ya ultrafiltration akenshi ntabwo ari umubare.Nubwo ibikoresho bya PES bigabanya adsorption idafite akamaro, poroteyine zimwe na zimwe, cyane cyane iyo zoroshye, zishobora kugira ibibazo.Urwego rwo guhuza rudasanzwe ruratandukana nuburyo bwa poroteyine zitandukanye.Poroteyine zirimo domeni zishyizwemo cyangwa hydrophobique birashoboka cyane guhuza bidasubirwaho ahantu hatandukanye.Kwiyitirira passivation hejuru yumuyoboro wa ultrafiltration centrifuge birashobora kugabanya igihombo cya protein adsorption hejuru ya membrane.Mu bihe byinshi, kwitegura inkingi mbere yo kwibanda kuri proteine ​​ya dilute irashobora kunoza igipimo cyo gukira, kubera ko igisubizo gishobora kuzuza imbuga za poroteyine zidafite ishingiro zigaragara kuri membrane no hejuru.Uburyo bwa passivation ni ukubanza gushiramo inkingi hamwe nubunini bwinshi bwumuti wa passivation mugihe cyamasaha arenga 1, koza inkingi neza namazi yatoboye, hanyuma ugashyiramo centrifuge rimwe hamwe namazi yatoboye kugirango ukureho burundu igisubizo cya passivation gishobora kuguma kuri firime. .Witondere kutareka firime ikuma nyuma ya passivation.Niba ushaka kuyikoresha nyuma, ugomba kongeramo amazi meza kugirango ugumane firime.

Ultrafiltration centrifuge imiyoboro ntishobora guhindurwa no gukoreshwa.Kubera ko igiciro cyumuyoboro umwe kidahenze, abantu benshi bagerageza kugikoresha - uburambe nugusukura hejuru ya membrane hamwe namazi yatoboye inshuro nyinshi hanyuma centrifuge ikabigira rimwe cyangwa kabiri.Umuyoboro muto ushobora kwinjizwa muri revers urashobora kwibizwa mumazi yatoboye hanyuma ugashyirwa muburyo butandukanye inshuro nyinshi, bizaba byiza.Irashobora gukoreshwa kurugero rumwe inshuro nyinshi, kandi irashobora gushirwa mumazi yatoboye mugihe idakoreshejwe, ariko hagomba gukumirwa kwanduza bagiteri.Ntukavange ingero zitandukanye.Abantu bamwe bavuga ko gushira muri 20% alcool na 1n NaOH (sodium hydroxide) bishobora kubuza gukura kwa bagiteri no kwirinda gukama.Igihe cyose ultrafiltration membrane yibasiye amazi, ntishobora kwemererwa gukama.Ariko, abandi bavuga ko bizasenya imiterere ya membrane.Ibyo ari byo byose, ababikora muri rusange ntibashyigikira kongera gukoresha.Gukoresha inshuro nyinshi bizahagarika ubunini bwa pore ya filteri ya membrane, ndetse bigatera n'amazi ava, bizagira ingaruka kubisubizo byubushakashatsi.