Ibisobanuro rusange byumuco w'akagari flask

Ibisobanuro rusange byumuco w'akagari flask

Umuco w'akagari bivuga uburyo bwigana ibidukikije byimbere muri vitro kugirango bibeho, bikure, byororoke kandi bikomeze imiterere n'imikorere yabwo.Imico itandukanye ikoreshwa mumico ya selile irakenewe mumico y'utugari, muri yo icupa ry'umuco w'akagari niryo rikoreshwa cyane.

Imiterere yumuco wumudugudu flask ni kare kandi icyuho ni kinini.Igishushanyo niki koroshya gusarura selile.Uruhande rwa flask mubusanzwe rwakonje, rworohereza uwukora kwandika.Ukurikije ibisobanuro, ibikoreshwa bisanzwe ni 25cm2, 75cm2, 175cm2, 225cm2, nibindi.Hariho ibintu bitandukanye byo gusaba ukurikije ibisobanuro bitandukanye.

Amacupa mato ya 25cm2 na 75cm2 akoreshwa cyane mugusubirana ingirabuzimafatizo no kwaguka kwinshi, cyane cyane mugihe cyambere cyumuco w'akagari.Irashobora kandi gukoreshwa nkububiko bwo kubika selile zitandukanye zitukura.Amacupa manini ya cm 175 na cm 225 akoreshwa cyane cyane mumico mito mito cyangwa imvugo ya eukaryotic.Imvugo ya poroteyine ni tekinoroji y’ibinyabuzima ikoresha za bagiteri, umusemburo, ingirabuzimafatizo, ingirangingo z’inyamabere cyangwa ingirabuzimafatizo kugira ngo zigaragaze poroteyine za gene zidasanzwe, kandi ni kimwe mu bintu by'ingenzi bigize ikoranabuhanga mu buhanga.

Flask yumuco w'akagari ikoreshwa cyane cyane kumuco w'utugingo ngengabuzima.Nubwo ibisobanuro bitandukanye bitandukanye mugukoresha, bigomba kuba byujuje ibisabwa kugirango imikurire ikure, nka nta DNase, nta enzyme ya RNA, nta endotoxine, nta soko ry’inyamaswa, kuvura TC hejuru, nibindi.

  Agasanduku k'umuco w'akagari ka Labio gafite ibintu bikurikira:

1.Ibikoresho byubuvuzi bwisugi polystyrene (PS), byerekana umucyo mwinshi kandi birwanya imiti

2.Kugaragaza ubuso bunoze hamwe nuburinganire bumwe bwashushanyijeho cap cap, hamwe na 0.22 μm hydrophobic filter yo guhanahana byoroshye

3.Gufata ijosi ryemerera gukora byoroshye spatula selile na pipettors

4.Ku gice cyanditseho ubukonje ku ijosi, no gutanga impamyabumenyi kumpande zombi gusudira ultrasonic ikoreshwa hagati yumupfundikizo na flask, ukirinda kumeneka nicyuma mubifata

5.Ibishushanyo mbonera byo kubika umwanya no kubika byoroshye

6.Yakozwe mubyumba 100.000 byicyumba gisukuye, kirimo DNase, RNase, pyrogen na endotoxic kubuntu

7.Umupaki kugiti cye, sterisile na irrasiyo, SAL 10-6

8.Biboneka mubushobozi butatu, TC ivurwa cyangwa itavuwe guhitamo.