Amakuru - Sobanura ibyiciro bya kashe ya PCR

Sobanura ibyiciro bya firime ya kashe ya PCR

Firime yo gufunga ifoto ya microplate (Yera) ni ubwoko bwibikoresho byimiti yibitabo byifashishwa mu gufunga microplate nka plaque ya enzyme na plaque ya PCR.Nyuma yo gufunga isahani hamwe niki gicuruzwa, guhumeka kwamazi mumyobo yisahani isobekeranye birashobora gukumirwa, kwanduza umusaraba hagati yumwobo birashobora kugabanuka, ikosa ryikigereranyo rirashobora kugabanuka, kandi ukuri kurashobora kunozwa.Irashobora gukoreshwa nk'icyapa gifunga ELISA, kumenya amabara atandukanye cyangwa kumenya fluorescence.

1. Filime isanzwe ya PCR:

Bikwiranye na PCR reaction, ikozwe muri polypropilene membrane

RNase / DNase na aside nucleic yubusa

Isahani yo gufunga biroroshye kandi ntabwo byoroshye kugorama

Ubushyuhe bwo gukora: - 40 ℃ - + 120 ℃

2. Fluorescent yuzuye PCR membrane ifunga:

Kubangikanya, kwifata rya autofluorescence, bikwiranye na fluorescence igereranya PCR

Birakwiriye kubisahani bitandukanye bya PCR, ntabwo byacitse

DNase / RNase na aside nucleic yubusa, anti DMSO

Isahani yo gufunga biroroshye kandi ntabwo byoroshye kugorama

Ubushyuhe bwo gukora - 70 ℃ - + 100 ℃

Filime yoroheje yoroheje, ifata ni urwego rwubuvuzi rukomeye.Ubunini bwa firime ifata ni 10um, ifite ubukungu kandi bworoshye kuyikoresha, kandi muri rusange ikoreshwa kubibaho rusange bya PCR.

3. Firime ya PCR ya aluminium

Ntabwo byoroshye byoroshye bya aluminiyumu, ibifata ni urwego rwubuvuzi rukomeye, rukwiriye kubikwa igihe kirekire

Ugereranije nizindi firime ya kashe ya aluminium, iyi firime ntabwo yoroshye kugorama iyo ikuwe ku isahani

Imikorere myiza yo kurwanya impumuro nziza, hafi nta guhinduka kwicyitegererezo, byoroshye gucumita

DNase / RNase na aside nucleic yubusa

Birakwiriye kubibaho bitandukanye bya PCR, harimo imbaho zifite impande zazamuye

4. Filime yoroheje yumuvuduko ukabije:

· Ikozwe murwego rwa firime ya polypropilene ibonerana hamwe nigice cya silicon ibonerana ishingiye kumuvuduko ukabije.

· Ubushyuhe: - 70 ℃ - 100 ℃

· Kanda kuri firime yoroheje, idafatanye nuruhu na gants, byoroshye gukora ubushakashatsi, kandi ntabwo bigira ingaruka kubisesengura

· Ntabwo ikora hamwe nicyitegererezo cyubushakashatsi, kandi ibisubizo byubushakashatsi birizewe

· Nta fluorescence ihita

Igihe cyo kohereza: Nzeri-30-2022