Nigute ushobora guhitamo isahani ya PCR kubikoreshwa muri laboratoire?
Ibyapa bya PCR mubisanzwe ni 96-umwobo na 384-umwobo, bigakurikirwa na 24-umwobo na 48.Igikoresho cya PCR cyakoreshejwe hamwe nimiterere ya porogaramu iri gukorwa bizagaragaza niba ikibaho cya PCR gikwiranye nubushakashatsi bwawe.None, nigute ushobora guhitamo ikibaho cya PCR cyibikoresho bya laboratoire neza?
1 types Ubwoko butandukanye bwijipo butagira ikibaho cyikariso kandi kibuze ikibaho gikikije.
Ubu bwoko bwa plaque reaction irashobora guhuzwa nuburyo bwinshi bwibikoresho bya PCR nibikoresho nyabyo bya PCR, ariko ntibikwiriye gukoreshwa byikora.
Isahani y-ijipo ifite impande ngufi zizengurutse isahani kandi itanga inkunga ihagije mugihe cyoherejwe.Ibyinshi mu Byakoreshejwe Biosystems PCR ikoresha igice cya skirt.
Ikibaho cyuzuye-ijipo PCR ifite ikibaho gikingira uburebure bwikibaho.Ubu bwoko bwibibaho bubereye ibikoresho bya PCR hamwe na module isohoka (ifasha gukora byikora), kandi irashobora guhuzwa neza kandi neza.Amajipo yuzuye nayo yongerera imbaraga imashini, bigatuma akoreshwa cyane mugukoresha urubuga rwa robo mumikorere yikora.
2 types Ubwoko butandukanye bwibice
Igishushanyo mbonera cyuzuye gikoreshwa mubikoresho byinshi bya PCR kandi biroroshye gufunga no gutunganya.
Igishushanyo mbonera cya convex gifite imiterere ihuza neza nibikoresho bimwe na bimwe bya PCR (nk'ibikoresho bikoreshwa na Biosystems PCR), bifasha kuringaniza umuvuduko w'ubushyuhe budakenewe adapteri, bigatuma ihererekanyabubasha ryiza hamwe nibisubizo byizewe byubushakashatsi.
Isahani ya PCR irashobora gutanga amabara atandukanye kugirango yorohereze itandukaniro ryerekanwa no kumenya ingero, cyane cyane mubushakashatsi bwimbitse.Nubwo ibara rya plastike ridafite ingaruka ku kwongera ADN, birasabwa cyane gukoresha ibikoresho bya pulasitiki byera cyangwa ibikoreshwa bya pulasitike bikonje kuruta ibyakoreshejwe mu mucyo mugihe washyizeho reaction ya PCR yibihe nyabyo PCR kugirango igaragaze neza kandi neza.
4 imyanya itandukanye
Gukata inguni ni inguni yabuze ya plaque ya PCR, biterwa nigikoresho cyo guhuza.Chamfer irashobora kuba kuri H1, H12 cyangwa A12 ya plaque 96, cyangwa A24 ya plaque 384.
5 format Imiterere ya ANSI / SBS
Kugirango uhuze na sisitemu zitandukanye zikoresha zikoresha sisitemu nyinshi zinjira cyane, ubuyobozi bwa PCR bugomba kubahiriza ishyirahamwe ry’ubuziranenge bw’Abanyamerika (ANSI) hamwe na Sosiyete ishinzwe ubumenyi bw’ibinyabuzima na molekuline (SBS), ubu ikaba ifitanye isano na Laboratoire Automation na Ishyirahamwe ryerekana (SLAS).Ikibaho gihuye na ANSI / SBS gifite ubunini busanzwe, uburebure, umwanya wu mwobo, nibindi, bifasha gutunganya byikora.
6 、 Uruhande
Hariho uruziga ruzamuye ruzengurutse umwobo.Igishushanyo gishobora gufasha gufunga kashe ya plaque kugirango wirinde guhumeka.
7 Mark
Mubisanzwe ni ikimenyetso cyimyandikire yazamuye hamwe cyera cyangwa umukara wandikishijwe intoki muburyo bwambere kugirango ubone byoroshye.
Igihe cyo kohereza: Gashyantare-10-2023