Nigute ushobora gukoresha umuyoboro wa cryopreservation mubuhanga kandi neza
Gukoresha umuyoboro wa cryopreservation ni siyanse, ntabwo ari trilogy yoroshye nko gufungura ikigega cya azote, kuyishyira mu muyoboro wa kirisiti no gufunga ikigega cya azote.Gukoresha siyansi kandi yukuri gukoresha tebes zirashobora kwirinda gutakaza ingero no kurinda umutekano wibizamini.
Gukoresha umuyoboro wa cryopreservation ni siyanse, ntabwo ari trilogy yoroshye nko gufungura ikigega cya azote, kuyishyira mu muyoboro wa kirisiti no gufunga ikigega cya azote.Gukoresha siyansi kandi yukuri gukoresha tebes zirashobora kwirinda gutakaza ingero no kurinda umutekano wibizamini.
Gukonjesha umuyoboro: intambwe zo gukonjesha
Koza selile ukoresheje igisubizo gishyushye cya PBS, unywe igisubizo, hanyuma utwikire selile igisubizo kirimo trypsin na EDTA (igicucu cyoroshye kirahagije, kandi kwibumbira hamwe kwa trypsin na EDTA bigomba kugenwa ukurikije umurongo w'akagari).
Shyiramo selile kuri 37 ℃ muminota 3-5.
Ingirabuzimafatizo zimaze kuva hasi, incubation irarangiye, hongeweho uburyo burimo serumu, kandi selile zihagarikwa buhoro buhoro hamwe na pipeti.
Centrifuge guhagarika selile (500 xg, 5 min) no guhagarikwa hamwe nuburyo burimo serumu.
Kubara Akagari.
Centrifuge ihagarikwa rya selile (500 xg, iminota 5), ikureho ndengakamere, hanyuma uhagarike ingirabuzimafatizo hamwe na serumu yubunini bukwiye.
Kuvanga selile hamwe na cryopreservation igisubizo (60% giciriritse, 20% ya serumu y'inda y'inda, 20% DMSO) mukigereranyo cya 1: 1, hanyuma ubyohereze muri Cryo STM cryopreservation tube.Ubucucike bw'utugingo ngengabuzima twakonje ni 1-5 × 106 ibice / ml.
Cryo irimo selile STM cryopreservation tube irasabwa gukonja ku gipimo cya - 1 K / min, kandi umuyoboro wa cryopreservation urashobora gushyirwa mubintu birimo isopropanol kuri - 70 ℃.Niba umuyoboro wa Cryo STM ubika izindi ngero, zishobora gushyirwa kuri - 20 ℃, - 70 ℃ cyangwa icyiciro cya gaze ya azote yuzuye.Kugirango umenye neza ko icyitegererezo cyakonjeshejwe kimwe, 4 mL na 5 mL Cryo Umuyoboro wa sTM ukenera gushyirwa muri firigo kuri - 20 ℃ ijoro ryose, hanyuma ukoherezwa mu cyiciro cya gaze ya - 70 ℃ cyangwa azote yuzuye.
Noneho ohereza Cryo.sTM cryopreservation tube mumazi ya azote.Kugirango wirinde umwanda (nka mycoplasma) hamwe no gutekereza ku mutekano, nyamuneka shyira umuyoboro wa Cryo.sTM wo mu bwoko bwa cryopreservation mu cyiciro cya gaze ya azote yuzuye, ntabwo iri mu cyiciro cy’amazi.
Nigute ushobora gukoresha umuyoboro wa cryopreservation mubuhanga kandi neza?Isosiyete yacu igizwe ninzobere zifite inganda nuburambe ku isoko kugirango zitange ibicuruzwa mubuzima bwubumenyi bwa siyanse na serivisi kubashakashatsi ba siyanse.Ntishobora gusa kuba yujuje ibyifuzo byihariye byabakiriya ba R&D kubwoko bwibicuruzwa no gupakira, ariko kandi byujuje ibyifuzo byinganda zibyara umusaruro mubyiciro byose kuva murwego ruto, ruciriritse kugeza ku bicuruzwa binini.
Igihe cyo kohereza: Ugushyingo-25-2022