Ikoranabuhanga ryumuco winyamanswa / ibimera nuruhererekane rwibikorwa bya biotechnologie bikorerwa ku ngirabuzimafatizo yitaruye cyangwa protoplasts kurwego rwa selile kubwintego runaka.Harimo kwigunga, umuco, kuvugurura hamwe nuruhererekane rwibikorwa bifitanye isano.Kubijyanye no gutanga umusaruro wingirakamaro, ahanini bivuga inzira yo kubyara ibintu bifatika binyuze mumuco wo guhagarika ingirabuzimafatizo mubihe bidasanzwe.
Shandong Labiosterile selile triangular shake flask yagenewe byumwihariko kumuco wingirabuzimafatizo 293, CHO nizindi selile.Shake flask ifite epfo na ruguru hamwe nigifuniko gihumeka.Irashobora gukoreshwa mugutezimbere kwiterambere rito, intambwe ku yindi amplification hamwe nizindi nzego z'umuco.Igifuniko gihumeka gifite firime 0.2μm ihumeka, ihumeka kandi ntishobora kwinjizwa mumazi, ishobora gukumira neza kwinjiza mikorobe, kwirinda kwanduza, no kwemeza guhanahana gaze, bigatuma selile zikura neza.
Iyo ukoresheje flask ya mpandeshatu, nibyiza kugenzura ingano yumuco wongeyeho 20 kugeza 30% yubunini bwa flask.Hano hari imirongo isobanutse yo gutanga impamyabumenyi kuri mpandeshatu ya shake flask kugirango byoroshye gukoreshwa.Umuvuduko wo kuzunguruka wa shaker urasabwa kugenzurwa kuri 75 ~ 125RPM, ushobora guhinduka ukurikije uko ibintu bimeze.
Igihe cyo kohereza: Ukuboza-21-2023