Icyitegererezo cyo gukusanya, kubika no gutwara ibisabwa kubushakashatsi busanzwe

Icyitegererezo cyo gukusanya, kubika no gutwara ibisabwa kubushakashatsi busanzwe

1. Gukusanya no kubungabunga ingero z’indwara:

Section Igice cyakonjeshejwe: Kuraho ibice bikwiye hanyuma ubibike muri azote yuzuye;

Igice cya paraffin: Kuraho ibice bikwiye hanyuma ubibike muri 4% paraformaldehyde;

Igicapo cyo guhamagara: Ishusho ya selile yashyizwe muri 4% paraformaldehyde muminota 30, hanyuma isimbuzwa PBS hanyuma yibizwa muri PBS ibikwa kuri 4 ° C.

2. Gukusanya no kubungabunga ingirabuzimafatizo ya biologiya:

Ush Tissue nshya: Kata urugero hanyuma ubibike muri azote yuzuye cyangwa firigo -80 ° C;

Ingero za paraffin: Bika ku bushyuhe bwicyumba;

BloodIcyitegererezo cy'amaraso yose: Fata umubare ukwiye w'amaraso yose hanyuma wongereho EDTA cyangwa heparin anticoagulation umuyoboro w'amaraso;

Ingero z'amazi meza: centrifugation yihuta yo gukusanya imyanda;

EllGuhamagarira ingero: Ingirabuzimafatizo ziterwa na TRizol kandi zibikwa muri azote yuzuye cyangwa firigo -80 ° C.

3. Gukusanya no kubika urugero rwa protein igerageza:

Ush Tissue nshya: Kata urugero hanyuma ubibike muri azote yuzuye cyangwa firigo -80 ° C;

BloodIcyitegererezo cy'amaraso yose: Fata umubare ukwiye w'amaraso yose hanyuma wongereho EDTA cyangwa heparin anticoagulation umuyoboro w'amaraso;

EllGuhamagarira ingero: Ingirabuzimafatizo zuzuyemo igisubizo cya lysis selile hanyuma zikabikwa muri azote yuzuye cyangwa -80 ° C.

4. Gukusanya no kubika ELISA, radioimmunoassay, hamwe nubushakashatsi bwibinyabuzima:

SampleSerum (plasma) icyitegererezo: Fata amaraso yose hanyuma uyongere kuri tube ya procoagulation (anticoagulation tube), centrifuge saa 2500 rpm muminota igera kuri 20, gukusanya supernatant, hanyuma ubibike muri azote yuzuye cyangwa muri firigo -80 ° C.

SUrine sample: centrifuge icyitegererezo kuri 2500 rpm muminota 20, hanyuma ubibike muri azote yuzuye cyangwa -80 ° C ya firigo;reba kuri ubu buryo bwa thoracic na ascites fluid, cerebrospinal fluid, na alveolar lavage fluid;

EllGuhamagarira ingero: Mugihe ubonye ibice byasohotse, centrifuge ingero za 2500 rpm muminota 20 hanyuma ubibike muri azote yuzuye cyangwa firigo -80 ° C;mugihe umenye ibice bigize ingirabuzimafatizo, Koresha ihagarikwa rya selile hamwe na PBS hanyuma uhagarike kandi ushonze inshuro nyinshi kugirango usenye selile kandi urekure ibice bigize ingirangingo.Centrifuge saa 2500 rpm muminota igera kuri 20 hanyuma ukusanyirize hamwe ndengakamere nkuko byavuzwe haruguru;

SamplesIcyitegererezo: Nyuma yo guca ingero, ubipime hanyuma ubihagarike muri azote yuzuye cyangwa firigo -80 ° C kugirango ikoreshwe nyuma.

5. Icyegeranyo cya Metabolomics:

SUrine sample: Centrifuge icyitegererezo kuri 2500 rpm muminota 20 hanyuma ubibike muri azote yuzuye cyangwa firigo -80 ° C;reba kuri ubu buryo bwa thoracic na ascites fluid, cerebrospinal fluid, alveolar lavage fluid, nibindi.;

FterNyuma yo gukata icyitegererezo cya tissue, gipima hanyuma uhagarike muri azote yuzuye cyangwa firigo -80 ° C kugirango ikoreshwe nyuma;