Guhitamo icyapa cyumuco

Ibyapa byumuco wutugari birashobora kugabanywa hasi no kuzenguruka (U-shusho na V-shusho) ukurikije imiterere yo hepfo;Umubare wimyobo yumuco yari 6, 12, 24, 48, 96, 384, 1536, nibindi;Ukurikije ibikoresho bitandukanye, hari isahani ya Terasaki hamwe nicyapa gisanzwe cyumuco.Guhitamo byihariye biterwa n'ubwoko bw'utugingo ngengabuzima, ingano y'umuco isabwa n'intego zitandukanye zigerageza.

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Imiterere itandukanye yibyapa byumuco bifite imikoreshereze itandukanye.Ingirabuzimafatizo z'umuco zisanzwe zimeze neza, zikaba zorohewe no kwitegereza microscopique, hamwe n'ahantu hakeye kandi ugereranije n'umuco w'akagari ugereranije.Kubwibyo, mugihe ukora MTT nubundi bushakashatsi, isahani yo hasi irakoreshwa muri rusange, utitaye ko selile zifatanije nurukuta cyangwa zahagaritswe.Isahani yumuco wo hasi igomba gukoreshwa mugupima agaciro kinjira.Witondere cyane kubikoresho, hanyuma ushire akamenyetso "Umuco wa Tissue (TC) Uvuwe" kumuco w'akagari.

U-isahani ya U cyangwa V isa muri rusange ikoreshwa mubisabwa bidasanzwe.Kurugero, muri immunologiya, iyo lymphocytes ebyiri zitandukanye zivanze numuco, bakeneye guhura no guterana inkunga.Muri iki gihe, isahani U-isanzwe ikoreshwa kubera ko selile zizahurira mu ntera ntoya kubera ingaruka za rukuruzi.Isahani yumuco wo hasi irashobora kandi gukoreshwa mugeragezwa kwishyiriraho isotope, bisaba ibikoresho byo gukusanya ingirabuzimafatizo gukusanya umuco w'akagari, nka "umuco wa lymphocyte uvanze".Amasahani ya V akoreshwa kenshi mukwica selile no gupima ammunologue.Ubushakashatsi bwo kwica selile burashobora kandi gusimburwa nisahani U (nyuma yo kongeramo selile, centrifuge kumuvuduko muke).

(2) Itandukaniro riri hagati yisahani ya Terasaki nicyapa gisanzwe cyumuco

Isahani ya Terasaki ikoreshwa cyane mubushakashatsi bwa kristu.Igishushanyo mbonera cyibicuruzwa byoroshye kwitegereza no gusesengura imiterere.Hariho uburyo bubiri: kwicara no kumanika igitonyanga.Uburyo bubiri bukoresha ibicuruzwa bitandukanye.Crystal class polymer yatoranijwe nkibikoresho, nibikoresho bidasanzwe nibyiza byo kureba imiterere ya kristu.

Isahani yumuco w'akagari ikozwe ahanini mubikoresho bya PS, kandi ibikoresho bivurwa hejuru, bikaba byoroshye gukura no kwaguka kwakagari.Byumvikane ko, hari n'ibikoresho byo gukura bya selile planktonic, kimwe n'ubuso buke bwo guhuza.

(3) Itandukaniro riri hagati yicyapa cyumuco na selile Elisa

Isahani ya Elisa muri rusange ihenze kuruta isahani yumuco.Isahani y'utugari ikoreshwa cyane cyane mu muco w'akagari kandi irashobora no gukoreshwa mu gupima intungamubiri za poroteyine;Isahani ya Elisa ikubiyemo isahani yo gutwikiraho isahani, kandi muri rusange ntabwo ikeneye gukoreshwa mu muco w'akagari.Ikoreshwa cyane cyane mugutahura poroteyine nyuma yubudahangarwa bwimisemburo ifitanye isano na reaction, bisaba ibisabwa hejuru hamwe na label yihariye ya enzyme ikora igisubizo.

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Urwego rwamazi yumuco amazi yongewe kumasahani atandukanye ya orifice ntagomba kuba ndende cyane, mubisanzwe murwego rwa 2 ~ 3mm.Umubare wamazi ukwiye wa buri mwobo wumuco urashobora kubarwa muguhuza igice cyo hasi cyibyobo bitandukanye.Niba hiyongereyeho amazi menshi, guhanahana gaze (ogisijeni) bizagira ingaruka, kandi biroroshye kurengerwa mugihe cyimuka, bigatera umwanda.Ubucucike bwihariye bwimikorere biterwa nintego yubushakashatsi.