Intambwe zihariye z'umuco w'Akagari

1. Ibikoresho bisanzwe

1. Ibikoresho mucyumba cyo kwitegura

Imashini imwe itobora amazi, ikurura amazi abiri, ikigega cya acide, ifuru, guteka igitutu, akabati ko kubika (kubika ibintu bidafite isuku), akabati ko kubika (kubika ibintu bitavanze), ameza yo gupakira.Ibikoresho mucyumba cyo gutegura igisubizo: uburinganire bwa torsion nuburinganire bwa elegitoronike (gupima imiti), metero ya PH (gupima PH agaciro k'umuti wumuco), imashini ya magnetiki (gushiraho icyumba cyo gukemura kugirango gikemuke).

2. Ibikoresho byicyumba cyumuco

Ikigega cya azote yuzuye, akabati yo kubika (kubika sundries), itara rya fluorescent n itara rya ultraviolet, sisitemu yo gutunganya ikirere, firigo ikonjesha ubushyuhe (- 80 ℃), icyuma gikonjesha, silindiri ya dioxyde de carbone, kumeza kuruhande (kwandika inyandiko zipimisha).

3. Ibikoresho bigomba gushyirwa mucyumba cya sterile

Centrifuge (ikusanya selile), ultra-isuku ikora, microscope idahindagurika, CO2 incubator (umuco wo gukuramo), kwiyuhagira amazi, kwanduza ogisijeni eshatu hamwe na sterisizione, 4 ℃ firigo (gushyira serumu n'umuti wumuco).

2 operation Igikorwa cya Aseptic

（1） Guhindura icyumba cya sterile

1. Sukura icyumba cya sterile buri gihe: rimwe mucyumweru, banza ukoreshe amazi ya robine kugirango ukure hasi, uhanagura ameza, kandi usukure ameza, hanyuma ukoreshe 3 ‰ lysol cyangwa bromogeramine cyangwa 0.5% acide peracetike kugirango uhanagure.

2. Sterilisation ya CO2 incubator (incubator): banza uhanagure hamwe na 3 ‰ bromogeramine, hanyuma uhanagure hamwe na 75% alcool cyangwa 0.5% acide peracetike, hanyuma ucane itara rya ultraviolet.

3. Sterilisation mbere yubushakashatsi: fungura itara rya ultraviolet, steriseri ya ogisijeni itatu na sisitemu yo gutunganya ikirere muminota 20-30.

4. Kurandura nyuma yubushakashatsi: guhanagura ameza asukuye cyane, kumeza kuruhande hamwe na microscope ihindagurika hamwe na alcool 75% (3 ‰ bromogeramine).

Gutegura burundu abakozi ba laboratoire

1. Karaba intoki ukoresheje isabune.

2. Wambare imyenda yo kwigunga, ingofero zo kwigunga, masike na kunyerera.

3. Ihanagura amaboko n'umupira w'ipamba wa alcool 75%.

Kwerekana imikorere idasanzwe

1. Amacupa yose ya alcool, PBS, umuco wo hagati hamwe na trypsin yazanwe mubikorwa byogukora isuku bigomba guhanagurwa hamwe na 75% alcool hejuru yinyuma y icupa.

2. Kora hafi yumuriro wamatara yinzoga.

3. Ibikoresho bigomba guhindurwa mbere yo kubikoresha.

4. Ibikoresho (nk'ibicupa by'amacupa n'ibitonyanga) bikomeza gukoreshwa bigomba gushyirwa ahantu hirengeye, kandi bigomba gukomeza gushyuha mugihe cyo kubikoresha.

5. Ibikorwa byose bigomba kuba hafi y itara rya alcool, kandi ibikorwa bigomba kuba byoroshye kandi byukuri, kandi ntibigomba gukorwaho kubushake.Niba ibyatsi bidashobora gukora ku kigega cyamazi.

6. Mugihe wifuza ubwoko burenze bubiri bwamazi, witondere gusimbuza umuyoboro wokunywa kugirango wirinde kwanduza.

Reba igice gikurikira cyo kwanduza ibikoresho.