Itandukaniro riri hagati yicyapa cya ELISA, Icyapa cyumuco wumudugudu, isahani ya PCR, nicyapa cyimbitse

Itandukaniro riri hagati yicyapa cya ELISA, Icyapa cyumuco wumudugudu, isahani ya PCR, nicyapa cyimbitse

1. Isahani ya ELISA

Isahani ya ELISAMubisanzwe bikozwe muri polystirene, ni ubwoko bwibikoresho bikoreshwa bifatanije numusomyi wa microplate kubushakashatsi bwa immunoassay bujyanye na enzyme.Muri ELISA, antigene, antibodies nizindi biomolecules byamamazwa hejuru ya microplate hakoreshejwe uburyo butandukanye, hanyuma bigakorwa hamwe nicyitegererezo cyapimwe hamwe na enzyme yanditseho antigen cyangwa antibody muburyo butandukanye, hanyuma bikamenyekana numusomyi wa microplate.

Icyapa cy'umuco w'akagari

Ibyapa byumucozikoreshwa mu gukura selile cyangwa bagiteri.Hano hari imyobo 6, imyobo 12, imyobo 24, imyobo 48 nu 96.Irasa na plaque ya microtiter ibonerana, ariko ikoreshwa ryayo riratandukanye cyane.Ongeramo umubare ukwiye wumuco uciriritse kumariba yisahani yumuco, hanyuma uhindure umuco utugingo mubidukikije.Ibyapa byumuco rusange birasa-hasi, bikwiranye numuco wo guhagarika ingirabuzimafatizo hamwe nuduce, kandi hariho na U-U-U na U-V.Nyuma yo guhindura isura yo hejuru, irashobora gutuma igira umuco wimikorere yimikorere nimikorere yo gukura.Ibikoresho ni polystirene.

Isahani yumuco utugari ikoreshwa cyane cyane mumico y'utugari kandi irashobora no gukoreshwa mugupima intungamubiri za poroteyine;mugihe cyo kugerageza kwinjiza kwizuba ryumucyo ugaragara, ibyapa byinshi-96 neza neza birashobora gukoreshwa.Ariko, mugihe ugerageza neza-uburebure bwihariye nuburebure bwihariye, kugirango wirinde ingaruka ziterwa no kwinjiza plaque ya orifice, hagomba gukoreshwa microplate idasanzwe.

3. Icyapa cya PCR

UwitekaIcyapa cya PCRikoreshwa mubikoresho bya PCR, bikaba kimwe no gukoresha plaque ya microplate hamwe numusomyi wa microplate.Ikoreshwa nkicyiciro gikomeye gitwara, yemerera icyitegererezo kunyuramo PCR muriyo, hanyuma igakoresha igikoresho cya PCR kugirango tumenye.Mubyukuri, tubivuze mu buryo bworoshye, isahani ya PCR nuruvange rwimiyoboro myinshi ya PCR, mubisanzwe amariba 96.Mubisanzwe bikozwe mubikoresho bya PP.

4. Isahani yimbitse

Microplates nka microplate na plaque ya PCR irashobora gukoreshwa nka plaque ya microwell kuko ingano ya buri riba ari nto cyane.Hano hari isahani muri laboratoire ifite umwobo muremure witwaIbyapa Byimbitse.Ikozwe mubikoresho bya polymer PP kandi ifite imiti ihuje neza, irashobora gukoreshwa mukubika ibisubizo byinshi bya polar organique, acide acide na alkaline nibindi bisukari bya laboratoire.