Koresha Uburyo no Kwirinda Gukonjesha Tube
Mu bushakashatsi bwa mikorobi, hakoreshwa ibikoresho bimwe byubushakashatsi, ni ukuvuga umuyoboro wa cryopreservation.Ariko, bitewe nuburyo butandukanye, ingaruka ziratandukanye cyane.Kubera iyo mpamvu, kuri ubu, laboratoire nyinshi zo mu Bushinwa zikora imiyoboro yo kubika za bagiteri ubwazo, ibyo bikaba bitongera imbaraga mu kazi gusa, ahubwo nanone bitewe n’imiterere y’ibihe bitandukanye, ingaruka zo kubungabunga bagiteri ntizihora zishimishije.
Niyo mpamvu, birakenewe kumenya uburyo bwo gukoresha nuburyo bumwe na bumwe bwo kwirinda umuyoboro wa cryopreservation, kugirango ugire uruhare runini.
1.Uburyo bwo gusaba
1).Iyo ukoresheje umuyoboro wo kubika ibintu kugirango ubike ingero, birasabwa cyane gushyira umuyoboro wa kirisiti mu cyuka cya azote yuzuye cyangwa firigo kugirango ubike.Niba umuyoboro wa cryopreservation ubitswe muri azote yuzuye, haribishoboka ko azote yuzuye izinjira mumiyoboro ya cryopreservation.Mugihe cyo gukira, gazi ya azote yamazi bizatuma habaho ubusumbane bwumuvuduko wimbere ninyuma, ibyo bikaba bishoboka cyane ko umuyoboro wa kiropreservation uturika, kandi ufite ingaruka mbi kubinyabuzima.
2).Koresha umuyoboro wa cryopreservation kugirango ubyuke, kandi ukoreshe ibikoresho byo kurinda umutekano mugihe cyose.Birasabwa kwambara imyenda ya laboratoire, gants ya pamba no gukorera ku ntebe ya laboratoire itekanye.Niba bishoboka, nyamuneka kwambara amadarubindi cyangwa ingabo yo mu maso.Nkuko ubushyuhe bwimbere mu cyi buzaba burenze ubw'itumba, nyamuneka witonde.
3).Mugihe cyo kubika utugingo ngengabuzima, ubushyuhe bukonje bwigitereko bugomba kuba bumwe.Gukonjesha kutaringaniye bizatera urubura rwinshi, ruzabuza kwanduza ubushyuhe bwamazi kumpande zombi, bityo bikabyara umuvuduko mwinshi uteje akaga kandi byangiza umuyoboro ukonjesha.
4).Umubare w'icyitegererezo cyahagaritswe ntushobora kurenza urugero ntarengwa rw'akazi rusabwa n'umuyoboro wafunzwe.
2. Ibintu bikeneye kwitabwaho
1).Gukonjesha ibidukikije
Imiyoboro idakoreshwa irashobora kubikwa mubushyuhe bwicyumba cyangwa 2-8 ℃ mumezi 12;Umuyoboro watewe no guterwa urashobora kubikwa kuri - 20 ℃ kandi bikagira ingaruka nziza zo kubika amata mu mezi 12;Umuyoboro wanduye urashobora kubikwa kuri - 80 ℃, kandi umutwaro urashobora kubikwa neza mugihe cyamezi 24.
2).Gukonjesha igihe cyo kubika
Imiyoboro idakoreshwa irashobora kubikwa mubushyuhe bwicyumba cyangwa 2-8 ℃;Umuyoboro watewe urukingo ugomba kubikwa kuri - 20 ℃ cyangwa - 80 ℃.
3).Intambwe yo gukora yo gukonjesha
Fata imico mishya mumico ya bagiteri itunganijwe kugirango utegure ihagarikwa rya bagiteri hamwe n’umuvuduko uri hagati ya 3-4 ya McDonnell yo gutera inshinge no kubika imiyoboro;Komeza umuyoboro wo kubungabunga hanyuma uyihindukire inyuma inshuro 4-5 kugirango bagiteri zivemo, nta kuzunguruka;Shira umuyoboro wo kubika muri firigo kugirango ubungabunge (- 20 ℃ - 70 ℃
Igihe cyo kohereza: Ugushyingo-25-2022